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Feasibility validation and condition optimization utilizing fluorescence. <t>(A)</t> <t>Native-PAGE</t> gel image for the CHA reaction steps. Lane 1–7: H1, H2, T, H1 + T, H2 + T, H1 + H2, H1 + H2 + T. (B) Fluorescence monitoring of the CHA reaction progress was performed at 37 °C with 60 cycles and 30 s per cycle. (C) S/N ratio and fluorescence value of experimental and control groups at various temperatures after 30 min of reaction. (D) S/N ratio and fluorescence value of experimental and control groups at various H1:H2 ratios after 30 min of reaction at 35 °C. Data with error bars are represented as means ± standard deviation (n = 3).
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Feasibility validation and condition optimization utilizing fluorescence. <t>(A)</t> <t>Native-PAGE</t> gel image for the CHA reaction steps. Lane 1–7: H1, H2, T, H1 + T, H2 + T, H1 + H2, H1 + H2 + T. (B) Fluorescence monitoring of the CHA reaction progress was performed at 37 °C with 60 cycles and 30 s per cycle. (C) S/N ratio and fluorescence value of experimental and control groups at various temperatures after 30 min of reaction. (D) S/N ratio and fluorescence value of experimental and control groups at various H1:H2 ratios after 30 min of reaction at 35 °C. Data with error bars are represented as means ± standard deviation (n = 3).
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Feasibility validation and condition optimization utilizing fluorescence. <t>(A)</t> <t>Native-PAGE</t> gel image for the CHA reaction steps. Lane 1–7: H1, H2, T, H1 + T, H2 + T, H1 + H2, H1 + H2 + T. (B) Fluorescence monitoring of the CHA reaction progress was performed at 37 °C with 60 cycles and 30 s per cycle. (C) S/N ratio and fluorescence value of experimental and control groups at various temperatures after 30 min of reaction. (D) S/N ratio and fluorescence value of experimental and control groups at various H1:H2 ratios after 30 min of reaction at 35 °C. Data with error bars are represented as means ± standard deviation (n = 3).
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Feasibility validation and condition optimization utilizing fluorescence. <t>(A)</t> <t>Native-PAGE</t> gel image for the CHA reaction steps. Lane 1–7: H1, H2, T, H1 + T, H2 + T, H1 + H2, H1 + H2 + T. (B) Fluorescence monitoring of the CHA reaction progress was performed at 37 °C with 60 cycles and 30 s per cycle. (C) S/N ratio and fluorescence value of experimental and control groups at various temperatures after 30 min of reaction. (D) S/N ratio and fluorescence value of experimental and control groups at various H1:H2 ratios after 30 min of reaction at 35 °C. Data with error bars are represented as means ± standard deviation (n = 3).
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Feasibility validation and condition optimization utilizing fluorescence. <t>(A)</t> <t>Native-PAGE</t> gel image for the CHA reaction steps. Lane 1–7: H1, H2, T, H1 + T, H2 + T, H1 + H2, H1 + H2 + T. (B) Fluorescence monitoring of the CHA reaction progress was performed at 37 °C with 60 cycles and 30 s per cycle. (C) S/N ratio and fluorescence value of experimental and control groups at various temperatures after 30 min of reaction. (D) S/N ratio and fluorescence value of experimental and control groups at various H1:H2 ratios after 30 min of reaction at 35 °C. Data with error bars are represented as means ± standard deviation (n = 3).
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Feasibility validation and condition optimization utilizing fluorescence. <t>(A)</t> <t>Native-PAGE</t> gel image for the CHA reaction steps. Lane 1–7: H1, H2, T, H1 + T, H2 + T, H1 + H2, H1 + H2 + T. (B) Fluorescence monitoring of the CHA reaction progress was performed at 37 °C with 60 cycles and 30 s per cycle. (C) S/N ratio and fluorescence value of experimental and control groups at various temperatures after 30 min of reaction. (D) S/N ratio and fluorescence value of experimental and control groups at various H1:H2 ratios after 30 min of reaction at 35 °C. Data with error bars are represented as means ± standard deviation (n = 3).
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Feasibility validation and condition optimization utilizing fluorescence. (A) Native-PAGE gel image for the CHA reaction steps. Lane 1–7: H1, H2, T, H1 + T, H2 + T, H1 + H2, H1 + H2 + T. (B) Fluorescence monitoring of the CHA reaction progress was performed at 37 °C with 60 cycles and 30 s per cycle. (C) S/N ratio and fluorescence value of experimental and control groups at various temperatures after 30 min of reaction. (D) S/N ratio and fluorescence value of experimental and control groups at various H1:H2 ratios after 30 min of reaction at 35 °C. Data with error bars are represented as means ± standard deviation (n = 3).

Journal: ACS Omega

Article Title: Instrument-Free, Low-Cost and Dual-System Detection for Norovirus Based on Self-Assembling DNA Circuitry

doi: 10.1021/acsomega.6c00618

Figure Lengend Snippet: Feasibility validation and condition optimization utilizing fluorescence. (A) Native-PAGE gel image for the CHA reaction steps. Lane 1–7: H1, H2, T, H1 + T, H2 + T, H1 + H2, H1 + H2 + T. (B) Fluorescence monitoring of the CHA reaction progress was performed at 37 °C with 60 cycles and 30 s per cycle. (C) S/N ratio and fluorescence value of experimental and control groups at various temperatures after 30 min of reaction. (D) S/N ratio and fluorescence value of experimental and control groups at various H1:H2 ratios after 30 min of reaction at 35 °C. Data with error bars are represented as means ± standard deviation (n = 3).

Article Snippet: Native PAGE gel, DNA marker, and loading buffer were ordered from Sangon Biotech Co., Ltd. (Shanghai, China).

Techniques: Biomarker Discovery, Fluorescence, Clear Native PAGE, Control, Standard Deviation